Journal: Frontiers in Immunology

Article Title: Epithelial-to-mesenchymal transition is an active process in the large airways of patients with asthma-COPD overlap and partially abrogated by inhaled corticosteroid treatment: a bronchoscopy endobronchial biopsy study

doi: 10.3389/fimmu.2025.1531279

Figure Lengend Snippet: Spearman correlation analysis between E-cadherin percent expression and epithelial macrophages per mm of the reticular basement membrane (RBM) from samples of ACO (A) , N-cadherin percent expression and epithelial macrophages per mm of RBM from samples of ACO (B) , S100A4 positive basal cells and epithelial macrophages per mm of RBM from samples of ACO (C) , and vimentin positive basal cells and epithelial macrophages per mm of RBM (D) from samples of ACO.

Article Snippet: Cigarette smoke extract (CSE) was prepared by bubbling smoke from one filter-less Kentucky research cigarette, 3R4F, which contains 9.5 mg of tar and 0.8 mg of nicotine, through 10 mL of Bronchial Epithelial Basal Medium (BEBM, Lonza) at a rate of one cigarette every 5 minutes ( ).

Techniques: Expressing, Membrane

Journal: Frontiers in Immunology

Article Title: Epithelial-to-mesenchymal transition is an active process in the large airways of patients with asthma-COPD overlap and partially abrogated by inhaled corticosteroid treatment: a bronchoscopy endobronchial biopsy study

doi: 10.3389/fimmu.2025.1531279

Figure Lengend Snippet: Relative expression changes of N-cadherin and E-cadherin in human-derived lung primary epithelial cells (HC and ACO patients) post induction with Th-2 cytokine IL-13, CSE and EMT activator TGF-β. # p<0.05, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

Article Snippet: Cigarette smoke extract (CSE) was prepared by bubbling smoke from one filter-less Kentucky research cigarette, 3R4F, which contains 9.5 mg of tar and 0.8 mg of nicotine, through 10 mL of Bronchial Epithelial Basal Medium (BEBM, Lonza) at a rate of one cigarette every 5 minutes ( ).

Techniques: Expressing, Derivative Assay